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rabbit polyclonal anti mitf antibody  (Danaher Inc)


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    Structured Review

    Danaher Inc rabbit polyclonal anti mitf antibody

    Rabbit Polyclonal Anti Mitf Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+mitf/pmc11034927-15-0-5
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti mitf antibody - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair"

    Article Title: Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.113282


    Figure Legend Snippet:

    Techniques Used: Recombinant, Plasmid Preparation, Software

    Related Articles

    Membrane:

    Article Title: Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos
    Article Snippet: Briefly, 40 μig of protein lysate obtained from treated B16 cells was electrophoresed and transferred to polyvinylidene difluoride membrane (Millipore, Germany). .. The membrane was treated individually with primary antibody including rabbit polyclonal anti-MITF, 1:1000 (ab 20663, Abcam, UK); rabbit polyclonal anti- TYR, 1:1000 (ab 180753, Abcam, UK); mouse monoclonal anti-TRP-1, 1:100 (ab 3312, Abcam, UK); rabbit polyclonal anti-TRP-2, 1:100 (ab 74073, Abcam, UK), and rabbit polyclonal anti-β-actin, 1:1,000 (ab 8227, Abcam, UK) at room temperature for 1 h with agitation before washing with 1× tris-buffered saline and Tween® 20 (TBST), pH 7.2 for three times. .. The membrane was then treated with secondary antibody including polyclonal goat anti-rabbit IgG-horseradish peroxidase(HRP), 1:1,000 (P0448, Dako, Denmark) and polyclonal goat anti-mouse IgG-HRP, 1:1,000 (P0447, Dako, Denmark) at room temperature for 1 h with gentle agitation.

    Saline:

    Article Title: Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos
    Article Snippet: Briefly, 40 μig of protein lysate obtained from treated B16 cells was electrophoresed and transferred to polyvinylidene difluoride membrane (Millipore, Germany). .. The membrane was treated individually with primary antibody including rabbit polyclonal anti-MITF, 1:1000 (ab 20663, Abcam, UK); rabbit polyclonal anti- TYR, 1:1000 (ab 180753, Abcam, UK); mouse monoclonal anti-TRP-1, 1:100 (ab 3312, Abcam, UK); rabbit polyclonal anti-TRP-2, 1:100 (ab 74073, Abcam, UK), and rabbit polyclonal anti-β-actin, 1:1,000 (ab 8227, Abcam, UK) at room temperature for 1 h with agitation before washing with 1× tris-buffered saline and Tween® 20 (TBST), pH 7.2 for three times. .. The membrane was then treated with secondary antibody including polyclonal goat anti-rabbit IgG-horseradish peroxidase(HRP), 1:1,000 (P0448, Dako, Denmark) and polyclonal goat anti-mouse IgG-HRP, 1:1,000 (P0447, Dako, Denmark) at room temperature for 1 h with gentle agitation.

    Incubation:

    Article Title: Polarized, Cobblestone, Human Retinal Pigment Epithelial Cell Maturation on a Synthetic PEG Matrix.
    Article Snippet: Subscriber access provided by UB + Fachbibliothek Chemie | (FU-Bibliothekssystem) ACS Biomaterials Science & Engineering is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Article Title: Matrigel and Activin A promote cell-cell contact and anti-apoptotic activity in cultured human retinal pigment epithelium cells.
    Article Snippet: Age-related macular degeneration (AMD) is a leading cause of blindness among the aging population.. Currently, replacement of diseased retinal pigment epithelium (RPE) cells with transplanted healthy RPE cells could be a feasible approach for AMD therapy.. However, maintaining cell-cell contact and good viability of RPE cells cultured in vitro is difficult and fundamentally determines the success of RPE cell transplantation.

    Article Title: Blunted epidermal L-tryptophan metabolism in vitiligo affects immune response and ROS scavenging by Fenton chemistry, part 1: Epidermal H2O2/ONOO(-)-mediated stress abrogates tryptophan hydroxylase and dopa decarboxylase activities, leading to low serotonin and melatonin levels.
    Article Snippet: Vitiligo is characterized by a progressive loss of inherited skin color.. The cause of the disease is still unknown.. To date, there is accumulating in vivo and in vitro evidence for massive oxidative stress via hydrogen peroxide (H2O2) and peroxynitrite (ONOO ) in the skin of affected individuals.



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    OriGene mitf
    RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated <t>STAT3</t> <t>(Y705),</t> total STAT3, <t>MITF,</t> SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).
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    Cell Signaling Technology Inc mitf rabbit polyclonal
    RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated <t>STAT3</t> <t>(Y705),</t> total STAT3, <t>MITF,</t> SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).
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    Image Search Results


    Journal: Cell reports

    Article Title: Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair

    doi: 10.1016/j.celrep.2023.113282

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal Anti-MiTF antibody , Abcam , Cat# ab122982; RRID:AB_10902226.

    Techniques: Recombinant, Plasmid Preparation, Software

    Mean number of cells per millimeter for each marker

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet: Mean number of cells per millimeter for each marker

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques:

    A subpopulation of immature melanocytes exists in the interfollicular epidermis that differentiates temporarily during radiation exposure Mean number of cells per millimeter in the basal layer (n = 15 patients). (A) MITF-positive melanocytes ( ) and Bcl-2-positive melanocytes ( ). (B) MITF-negative cells ( ) and Bcl-2-negative cells ( ) are morphologically characterized as melanocytes. Error bars represent SEM.

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet: A subpopulation of immature melanocytes exists in the interfollicular epidermis that differentiates temporarily during radiation exposure Mean number of cells per millimeter in the basal layer (n = 15 patients). (A) MITF-positive melanocytes ( ) and Bcl-2-positive melanocytes ( ). (B) MITF-negative cells ( ) and Bcl-2-negative cells ( ) are morphologically characterized as melanocytes. Error bars represent SEM.

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques:

    Number of negative epidermal melanocytes per millimeter and percentage of the total number melanocytes counted for each marker

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet: Number of negative epidermal melanocytes per millimeter and percentage of the total number melanocytes counted for each marker

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques: Control

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet:

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques: Recombinant, Plasmid Preparation, Software

    RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated STAT3 (Y705), total STAT3, MITF, SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).

    Journal: Biomedicines

    Article Title: RICTOR Affects Melanoma Tumorigenesis and Its Resistance to Targeted Therapy

    doi: 10.3390/biomedicines9101498

    Figure Lengend Snippet: RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated STAT3 (Y705), total STAT3, MITF, SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).

    Article Snippet: Membranes were probed with the following primary antibodies: Myc-Tag, RICTOR, ACTIN, p-AKT (S473), AKT, p-ERK, ERK, pSTAT3 (Y705), STAT3, SOX10, AXL (Cell Signaling Technology, Danvers, MA, USA), and MITF (OriGene Technologies Inc, Rockville, MD, USA).

    Techniques: Western Blot, Transfection, Plasmid Preparation, Control, Expressing